Human RECOMBINANT Renin (Crystallographic Grade)

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Item #: 510-11R

Details

Crystallographic Grade Recombinant Human RENIN bulk quantities from 10-500mg single lot sizes. customs fills for human recombinant renin available.

Crystallographic Grade Recombinant human pro-renin was expressed in HEK cells, converted to renin, an active enzyme was purified using peptide affinity chromatography.

Human recombinant Renin Activity was measured by the cleavage of a fluorescent peptide substrate (Arg-Glu(EDANS)-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys(dabcyl)-Arg) from Molecular Probes (Cat # R-2931) and Renin activity was detected in a microplate format using a SpectroMax Gemini microplate reader. recombinant human Renin activity was measured as ~100 FU/min/g protein at the Km. Recombinant human Renin protein concentration was determined using amino acid analysis

What is Human Renin?
Human Renin, also known as angiotensinogenase, is a circulating enzyme released mainly by juxtaglomerular cells in the JGA of the kidneys in response to low blood volume or low body NaCl content, mediated through the release of prostaglandins. Although human Renin has hormone-like actions, human Renin cleaves a protein precursor in the circulation rather than working on a cellular target.

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