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Item #: 102-90
EC No:
215-783-6
CAS No:
1405-69-2
Activity:
> 10 u/mg powder
Molecular Weight:
66kDa with subunit at 16kDa
Source:
Avian Egg White
Cat. No:
102-90
Form:
Lyophilized
Manufacturer Lee Biosolutions produces Avidin in research and bulk quantities. Avidin can be placed in the buffer of your choice.
Avidin is a tetrameric protein. In chicken egg white, avidin makes up approximately 0.05% of total protein (approximately 1.8 mg per egg). The tetrameric protein contains four identical subunits (homotetramer) each of which can bind to biotin (Vitamin B7, vitamin H) with a high degree of affinity and specificity.
Avidin's affinity for biotin is exploited in wide ranging biochemical assays, including western blot, ELISA, ELISPOT and pull-down assays. Avidin immobilized onto solid supports is also used as purification media to capture biotin-labelled protein or nucleic acid molecules. For example, cell surface proteins can be specifically labelled with membrane impermeable biotin reagent, then specifically captured using an avidin-based support.