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Item #: 300-20
CPK, GPT, LDH, GLDH, MDH: < 0.1%, Protease: < 0.002 U/mg, Ammonia: < 0.01 micromole/mg
Glutamate Oxaloacetate Transaminase
Porcine (Pig) Heart
GOT, SGOT, Aspartate Aminotransferase, ASAT, Aspartate Transaminase, AST
One unit will catalyze the transamination of one micromole of L-aspartate to alpha-ketoglutarate forming L-glutamate and oxaloacetate per minute at 37C and pH 7.8
~92,000 (Feliss and martinez-Carrion 1970)
>0.7 mg protein/mg (OD280, E0.1%=1))
>250 U/mg @ 37C (Dimension Clinical Analyzer)
Lyophilized from sodium phosphate and pyridoxal-5-phosphate, pH 7.0
Lee Biosolutions produces Porcine Heart Aspartate Transferase (AST), also known as Glutamate Oxaloacetate Transaminase (GOT) for research and diagnostic manufacturing uses.
Technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
There are two SGOT/AST isoenzymes are present in humans, they have high similarity. GOT1, the cytosolic isoenzyme derives mainly from red blood cells and heart. GOT2, the mitochondrial isoenzyme is predominantly present in liver.
Other Related Products:
300-10 - Glutamate Oxaloacetate Transaminase/Aspartate Transferase (GOT/AST) - Porcine Heart - Liquid
305-10 - Glutamate Oxaloacetate Transaminase/Aspartate Transferase (GOT/AST) - Human Heart - Lyophilized
305-11 - Glutamate Oxaloacetate Transaminase/Aspartate Transferase (GOT/AST) - Human Heart - Liquid
306-10 - Glutamate Oxaloacetate Transaminase/Aspartate Transferase (GOT/AST) - Human Liver - Lyophilized