Item#: 125-15 APOLIPOPROTEIN A1, Human

HUMAN APO A1 apolipoprotein(s) Bulk Quantities avialable in 100-1000mg single lot sizes. Custom Fills for Human APO A1 Lipoprotein available.

Human APO A1 apolipoprotein: Single polypeptide chain in 245 amino acids with high alpha-helical content, major structurual component of high density lipoproteins, cofactor for lecthin:cholesterol acyltransferase, levels in general are inversely related to risk of coronary artery disease in humans.

Human APO A1 apolipoprotein is isolated by ultracentrifugation and delipidated by organic solvents. Human APO A1 Lipoprotein is purified by ion-exchange chromatography.

Human APO A1 Lipoprotein is soluble in aqueous buffers at low concentrations (<50ug/mL) but due to amphipathic nature, Human APO A1 Lipoprotein will self-associate into oligomers at high concentrations in the absence of chaotrupes (urea, guanidine hydrochloride).

Our Human APO A1 apolipoprotein Sterile Filtered through a .22 micron hydrophilic filter.

Apo-AI comprises ~70% of the protein moiety in HDL. It is a single polypeptide chain consisting of 245 amino acids with glutamic acid as the C-terminal residue and aspartic acid as the N-terminal residue. The molecular mass is reported to be 28.3 kDa. The protein is made up of one major isoform (pI 5.6) and two minor isoforms (pI 5.53 and 5.46). Apo-AI shows a high content of á-helix structure. The amphipathic regions in the á-helix structure seem to be responsible for lipid binding capacity. In aqueous solution, Apo-AI shows self-association with minor conformation change. Apo-AI activates lecithin-cholesterol (LCAT) acyltransferase, which is responsible for cholesterol esterification in plasma.