Human RECOMBINANT LYSOZYME

• High Purity Proteins
• Recombinant Proteins
• Antibodies
• Biologicals
• Fine Biochemicals
• Reagents

Item #: 421-10R

Details

Recombinant human lysozyme is expressed and purified from an animal free system.

Bulk quantities available.
Lysozyme lyses the peptidoglycan polymer typicall found in most bacterial cell walss.

Recombinant lysozyme has identical amino acid sequences as the native lyosozyme. In lysing bacterial cells, recombinant lysozyme is added to the cell extraction buffer at a range of 0.05 to 0.5 mg/mL (7000 to 70000 U/ml)

Typically, the extraction buffer contains 100mM tris HCL, 2mM EDTA, 0.05% trion I-100, pH 8.0. The cell paste is suspended in extaction buffer and incubated for at least 15 minutes at room temperature. Alternatively, Triton X-100 at 1% and PMSF at 1mM can be added separately and the lysis solution is further incubated for at least 15 minutews at 37C. The lysed cell suspension is clarified by centrifugation at 20,000 g for at least 15 minutes. The extraction conditions and concentration of recombinant lyosozyme needed for optiomal cell lysis and protein recovery depends on various factors.

Cell Lysis
Not only does lysozyme have very important clinical implications but it is also a useful tool in molecular biology. The ability of lysozyme to break down bacterial cell walls (cell lysis) is utilized by molecular biologists. Cell lysis is necessary to extract recombinant proteins or DNA from bacterial cells. Human lysozyme is preferred to chicken lysozyme because it is up to four times more active. Also, the activity of chicken lysozyme has high lot-to-lot variation. Many recombinant protein production systems prefer an animal-free lysozyme to avoid the risk of infectious disease transmission.

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