Catalogue Number: 400-10
Product Name Lipase
Source Porcine Pancreas
Catalogue Number 400-10
Purity Purified
Form Lyophilized
Activity > 100 U/mg (Dimension® Clinical Chemistry System)
Unit Definition One unit will catalyze the hydrolysis of one micromole per minute of 1, 2-o-dilauryl-rac-glycero-3-glutaric acid-(6\'-methylresorufin) ester in the presence of colipase, bile salt, and calcium chloride at 37°C
Protein > 0.3 mg protein/mg (Coomassie)
Specific Activity > 300 U/mg protein
Contaminants a-Amylase: < 0.6%
Protease: < 0.01%
Cholesterol Esterase: <1%
Ammonia: < 0.02 micromole/mg
Solubility Opalescent, light yellow to yellow solution (10 mg/mL saline)
Formulation Lyophilized from saline and calcium chloride, pH 7.0.
Appearance Off-white to brown powder
Reconstitution > 1 mg/mL in tris buffered saline, 1 mM calcium chloride, 1% BSA, pH 8.0 is recommended.
Related Products 122-10 - α-Amylase from porcine pancreas
197-25 - Colipase from porcine pancreas
188-10 - Cholesterol Esterase from porcine pancreas
Storage -20°C
Recertification 3 years
Synonyms Triacylglycerol Acylhydrolase,Triacylglycerol lipase, PPL
Molecular Weight ~48,000
CAS Number 9001-62-1
E.C. Number

Custom preparations, technical support, bulk quantities and aliquoting available, email for more details. Bulk quantities from 1-50 million unit single lot sizes. We are bulk manufacturers of diagnostic grade human and animal pancreatic lipase for research and clinical diagnostic manufacturers.

Lipase is a water soluble enzyme that catalyzes the hydrolysis of ester bonds in water insoluble, lipid substrates and is a glycoprotein. Lipase (triacylglycerol acyl hydrolase) fulfills a key function in dietary fat absorption by hydrolysing triglycerides into diglycerides and subsequently into monoglycerides and free fatty acids. Pancreatic enzymes have been most studied as part of the treatment for Celiac Disease.

Use: For investigation of pancreatic disorders, pancreatitis, acute and chronic renal disease.

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