Alkaline Phosphatase (ALP)
Catalogue Number: 486-10
Description
| Product Name | Alkaline Phosphatase |
| Source | Human Liver |
| Catalogue Number | 486-10 |
| Purity | Purified |
| Form | Liquid |
| Activity | > 25 U/mL (Dimension® Clinical Chemistry System) |
| Unit Definition | One unit will catalyze the hydrolysis of one micromole of p-nitrophenyl phosphate per minute at 37°C and pH 10.35 in the presence of 2-amino-2-methyl-1-propanol. |
| Protein | > 5 mg protein/mL (Coomassie) |
| Specific Activity | Reported in U/mg protein |
| Formulation | Suspension in 3.1 M ammonium sulfate with tris chloride, magnesium and zinc, pH 7.4. |
| Appearance | Tan to off-white suspension |
| Recovery | Centrifuge briefly to assure complete recovery. Mix thoroughly before sampling. |
| Storage | 2-8°C |
| Recertification | 2 years |
| CAS Number | 9001-78-9 |
| E.C. Number | 3.1.3.1 |
| Synonyms | ALP, HL-ALP |
| Gene | ALPL |
| Gene ID | 249 |
Applications
Overview
Lee Biosolutions is the leading supplier of Human Liver Alkaline Phosphatase (ALP) for life science, laboratory, research and diagnostic manufacturing uses.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
Alkaline Phosphatase (ALP) is a hydrolase enzyme responsible for removing phosphate groups in the 5- and 3- positions from many types of molecules, including nucleotides, proteins, and alkaloids.
In humans, alkaline phosphatase is present in all tissues throughout the entire body, but is particularly concentrated in liver, bile duct, kidney, bone, and the placenta. The optimal pH for the enzyme activity is pH=10 in standard conditions.
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