Tropomyosin (TPM)

Catalogue Number: 545-10
Product Name Tropomyosin
Source Human Heart
Catalogue Number 545-10
Purity > 95% (SDS-PAGE)
Form Lyophilized
Protein > 1.0 mg protein/mg solid (A280, E=0.274)
Form Lyophilized from 5mM ammonium bicarbonate
Appearance White powder
Reconstitution Use a neutral pH buffer of users choice, avoiding extreme high and low pH.
Recertification 5 years
Storage -20°C
Molecular Weight ~70,000
E.C. Number

Lee Biosolutions is the leading supplier of Human Heart Tropomyosin (TPM) for medical research and diagnostic manufacturing.

Technical support, bulk quantities, and custom buffers and aliquots are available, email for more details.

TROPOMYOSIN is an actin-binding protein that regulates actin mechanics. Tropmyosin is important, among other things, for muscle contraction. Tropomyosin, along with the troponin complex, associate with actin in muscle fibers and regulate muscle contraction by regulating the binding of myosin. In resting muscle, tropomyosin overlays the myosin binding sites on actin and is "locked" down in this position by troponin T (tropomyosin binding troponin) and troponin I (inhibitory troponin).

Upon release of calcium from the sarcoplasmic reticulum calcium binds to troponin C (calcium binding troponin). This "unlocks" tropomyosin from actin, allowing it to move away from the binding groove. Myosin heads can now access the binding sites on actin. Once one myosin head binds, this fully displaces tropomyosin and allows additional myosin heads to bind, initiating muscle shortening and contraction. Once calcium is pumped out of the cytoplasm and calcium levels return to normal, tropomyosin again binds to actin, preventing myosin from binding.

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545-10-100 $ 462.00 100 ug
545-10-500 $ 1,286.00 5 x 100 ug