Kappa Light Chain (KLC) B+F, anti-Human Polyclonal Antibody

Catalogue Number: GKBF-80A
Product Name Kappa Light Chain (KLC) B+F - Polyclonal anti-Human Antibody
Source Host Goat
Catalogue Number GKBF-80A
Form Liquid
Concentration 1 - 2 mg/mL (A280, E1mg/mL280nm = 1.35)
Purity Affinity Purified
Immunogen Highly purified kappa chains from human IgG
Specificity The affinity purified anti-kappa reacts with IgG kappa chains.
It does not react with IgG lambda chains. Suitable for IP, ELISA and blotting applications.
Form Solution in phosphate buffered saline pH 7.2 with 0.1% sodium azide
Storage 2-8°C
Recertification 2 years

Polyclonal anti-human Kappa procedure: Goats were immunized with highly purified human kappa chains from normal IgG. Following immunization in Freund's adjuvant the goats were bled, serum collected and the antiserum absorbed with a lambda containing immunoabsorbant. The anti kappa was isolated from an IgG kappa affinity column

The affinity purified anti kappa reacts with both bound and free kappa chains. It does not react with bound or free lambda chains by IEP and double diffusion analysis.

Western Blots 1:5,000-1-10,000 ELISA coating 1:100-1:500

Inquire on our custom polyclonal antibody production. We can host from any species and cross link to your requirements. Custom Polyclonal antibody concentrations available.

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GKBF-80A-1 $ 158.00 1 mg
GKBF-80A-5 $ 1,208.00 10 x 1 mg