Tryptase

Catalogue Number: 425-50
Description
Product Name Mast Cell Tryptase
Source Human Lung
Catalogue Number 425-50
Purity > 96% (SDS-PAGE)
Form Liquid
Protein 1.0 - 5.0 mg/mL (BCA)
Appearance Clear, colorless solution
Related Products 550-21 - Troponin T from human heart
431-11 - Myoglobin from human heart
426-10 - Myeloperoxidase from human leukocytes
Storage -20°C
Recertification 2 years
Molecular Weight ~40,000 (Monomer)
CAS Number 97501-93-4
E.C. Number 3.4.21.59
Overview

Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.

Tryptase is a member of the serine protease S1 family. It is the predominant neutral protease of the mast cell granules. Within the mast cell granule, it exists as a heparin-stabilized active tetramer. Stabilization is a result of the high negative charge density of the glycosaminoglycan. This stabilization activity is observed with heparins with a MW greater than 6 kDa as well as other glycosaminoglycans such as dextran sulfate or chondroitin sulfates. Removal of heparin results in dissociation of the tetramer and inactivation of the enzyme. High concentrations of NaCl will result in the dissociation of heparin.

Tryptase is released from the mast cell as a result of the degranulation response during anaphylaxis. In addition, several tryptase genes and alleles (alpha, beta, gamma and delta) have been identified in various tissues and circulating in blood. Pro-beta-tryptase is thought to be the constituative circulating form in blood.

The biological function of tryptase is unknown. However it has been reported to catalyze the activation of Complement C3, convert prostromelysin to stromelysin (MMP-3), and cleave fibrinogen resulting in a loss of clottting potential. Tryptase also degrades fibronectin, calcitonin gene-related peptide, vasoactive intestinal peptide, and Kininogen.

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SKU PRICE (USD) UNIT QTY
425-50-1 Inquire 1 mg