Alanine Aminotransferase (ALT, GPT)
| Product Name | Alanine Aminotransferase |
| Source | Human Liver |
| Catalogue Number | 310-18 |
| Purity | Purified |
| Form | Liquid |
| Activity | > 50 U/mL (Dimension® Clinical Chemistry System) |
| Unit Definition | One unit will catalyze the transamination of one micromole per minute of L-alanine to α- ketoglutarate, forming L-glutamate and pyruvate, at 37°C and pH 7.4. |
| Protein | > 1 mg/mL (Coomassie) |
| Specific Activity | > 10 U/mg protein |
| Contaminants | ALP: < 0.1% CK: < 0.1% gGT: < 0.1% AST/SGOT: < 3% LDH: < 1% |
| Formulation | Suspension in tris-buffered, 1.8 M ammonium sulfate suspension, pH 7.5. |
| Storage | 2-8°C |
| Recertification | 2 years |
| Product Specificity | Liver damage or disease |
| Synonyms | Glutamate Pyruvate Transaminase, Alanine Transaminase, serum glutamic-pyruvic transaminase, SGPT, GPT, ALT, ALAT |
| Molecular Weight | ~100 kDa |
| UNSPSC code | 51131907 |
| CAS Number | 9000-86-6 |
| E.C. Number | 2.6.1.2 |
Lee Biosolutions is the leading producer of Human Liver Glutamate Pyruvate Transaminase/Alanine transaminase (GPT/ALT) for medical research and clinical diagnostic assays for diagnosis of liver disease.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
Elevated levels of Alanine Transaminase (ALT)often suggest the existence of other medical problems such as alcoholic or viral hepatitis, congestive heart failure, liver damage, biliary duct problems, infectious mononucleosis, or myopathy.
Human GPT/ALT is an enzyme that catalyzes the reversible transfer of an amino group from alanine to a-ketoglutarate. It is found in serum and in various bodily tissues but is most commonly associated with the liver. Also called Serum Glutamate Pyruvate Transaminase (SGPT) or Alanine Aminotransferase (ALAT).
The alanine aminotransferase (ALT) blood test is typically used to detect liver injury, and is often ordered in conjunction with Aspartate Aminotransferase (AST) or as part of a liver panel to screen for and/or help diagnose liver disease. AST and ALT are considered to be two of the most important tests to detect liver injury, although ALT is more specific than AST. Sometimes AST is compared directly to ALT and an AST/ALT ratio is calculated. This ratio may be used to distinguish between different causes of liver damage.
Lee Biosolutions, Inc
