Neutrophil Elastase (ELA-2)

Catalogue Number: 342-45
Description
Product Name Elastase
Source Human Neutrophils
Catalogue Number 342-45
Purity > 96% (SDS-PAGE)
Form Lyophilized
Protein Reported in mg/vial or mg protein/mg solid (Coomassie)
Activity Reported
Unit Definition One unit will hydrolyze one micromole of methoxy succinyl-alanine-alanine-proline-valine-p-nitroanilide per minute at 37°C and pH 7.5.
Specific Activity > 10 U/mg protein
Formulation Lyophilized from sodium acetate, pH 5.5.
Reconstitution > 1 mg protein/mL in a buffer such as tris buffered saline, pH 7.5. Further dilutions should include a carrier such as 1% BSA.
Solubility Clear, colorless to slightly hazy (saline)
Related Products 550-21 - Troponin T antigen from human heart
431-11 - Myoglobin antigen from human heart
426-10 - Myeloperoxidase antigen from human leukocytes
Storage 2-8°C
Recertification 3 years
Infectious Disease Testing Negative or non-reactive at the donor level for anti-HIV 1 and 2, anti-HCV, HBsAg, HCV NAT, HIV-1 NAT and syphilis by FDA approved methods.
Synonyms ELA-2, Elastase, HNE, NEG
CAS Number 9004-06-2
E.C. Number 3.4.21.37
Gene ELANE, ELA2, GE, HLE, HNE, NE, PMN-E, SCN1
Gene ID 1991
Accession No P08246
Overview

Lee Biosolutions is the leading producer of human neutrophil Elastase (NEG) for clinical research, life science and diagnostic manufacturing uses.

Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.

Human Neutrophils, also known as neutrophilic polymorphonuclear leukocytes, are the major class of white blood cell in human peripheral blood. They, have a multilobed nucleus and neutrophilic granules, and are phagocytes and have an important role in engulfing and killing extracellular pathogens.

The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate chains. Current studies show that both Cathepsin G and Neutrophil Elastase are key enzymes for tissue injury caused by activated neutrophils, such as occurs in Acute Lung injury.

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SKU PRICE (USD) UNIT QTY
342-45-0.1 $ 342.00 100 ug
342-45-1 $ 1,292.00 1 mg