Lipase
Product Name | Lipase |
Source | Porcine Pancreas |
Catalogue Number | 400-10 |
Purity | Purified |
Form | Lyophilized |
Activity | > 100 U/mg (Dimension® Clinical Chemistry System) |
Unit Definition | One unit will catalyze the hydrolysis of one micromole per minute of 1, 2-o-dilauryl-rac-glycero-3-glutaric acid-(6\'-methylresorufin) ester in the presence of colipase, bile salt, and calcium chloride at 37°C |
Protein | > 0.3 mg protein/mg (Coomassie) |
Specific Activity | > 300 U/mg protein |
Contaminants | a-Amylase: < 0.6% Protease: < 0.01% Cholesterol Esterase: <1% Ammonia: < 0.02 micromole/mg |
Solubility | Opalescent, light yellow to yellow solution (10 mg/mL saline) |
Formulation | Lyophilized from saline and calcium chloride, pH 7.0. |
Appearance | Off-white to brown powder |
Reconstitution | > 1 mg/mL in tris buffered saline, 1 mM calcium chloride, 1% BSA, pH 8.0 is recommended. |
Related Products | 122-10 - α-Amylase from porcine pancreas 197-25 - Colipase from porcine pancreas 188-10 - Cholesterol Esterase from porcine pancreas |
Storage | -20°C |
Recertification | 3 years |
Synonyms | Triacylglycerol Acylhydrolase,Triacylglycerol lipase, PPL |
Molecular Weight | ~48,000 |
CAS Number | 9001-62-1 |
E.C. Number | 3.1.1.3 |
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