Product Name Lipase
Source Porcine Pancreas
Catalogue Number 400-10
Purity Purified
Form Lyophilized
Activity > 100 U/mg (Dimension® Clinical Chemistry System)
Unit Definition One unit will catalyze the hydrolysis of one micromole per minute of 1, 2-o-dilauryl-rac-glycero-3-glutaric acid-(6\'-methylresorufin) ester in the presence of colipase, bile salt, and calcium chloride at 37°C
Protein > 0.3 mg protein/mg (Coomassie)
Specific Activity > 300 U/mg protein
Contaminants a-Amylase: < 0.6%
Protease: < 0.01%
Cholesterol Esterase: <1%
Ammonia: < 0.02 micromole/mg
Solubility Opalescent, light yellow to yellow solution (10 mg/mL saline)
Formulation Lyophilized from saline and calcium chloride, pH 7.0.
Appearance Off-white to brown powder
Reconstitution > 1 mg/mL in tris buffered saline, 1 mM calcium chloride, 1% BSA, pH 8.0 is recommended.
Related Products 122-10 - α-Amylase from porcine pancreas
197-25 - Colipase from porcine pancreas
188-10 - Cholesterol Esterase from porcine pancreas
Storage -20°C
Recertification 3 years
Synonyms Triacylglycerol Acylhydrolase,Triacylglycerol lipase, PPL
Molecular Weight ~48,000
CAS Number 9001-62-1
E.C. Number