Lipase (PPL)

Product Name Lipase
Source Porcine Pancreas
Catalogue Number 400-10
Purity Purified
Form Lyophilized
Activity > 240 U/mg (Dimension® Clinical Chemistry System)
Protein > 0.3 mg protein/mg (Coomassie)
Specific Activity > 300 U/mg protein
Contaminants a-Amylase: < 0.2%
Protease: < 0.01%
Cholesterol Esterase: <1%
Trypsin: < 2%
Ammonia: < 0.02 micromole/mg
Unit Definition One unit will catalyze the hydrolysis of one micromole per minute of 1, 2-o-dilauryl-rac-glycero-3-glutaric acid-(6\'-methylresorufin) ester in the presence of colipase, bile salt, and calcium chloride at 37°C
Form Lyophilized from saline and calcium chloride, pH 7.0
Recertification 3 years
Storage -20°C
Reconstitution > 1 mg/mL in tris buffered saline, 1 mM calcium chloride, 1% BSA, pH 8.0 is recommended.
Synonyms Triacylglycerol Acylhydrolase,Triacylglycerol lipase, PPL
Molecular Weight ~48,000
CAS Number 9001-62-1
E.C. Number 3.1.1.3