Creatine Kinase MB (CK-MB), Type I

Catalogue Number: 190-24-R1
Product Name Human Creatine Kinase MB
Source Recombinant
Catalogue Number 190-24-R1
Purity > 95% (SDS-PAGE)
Form Liquid
Expression System Pichia pastoris (Yeast)
Protein > 1.0 mg/mL (Coomassie)
Activity > 400 U/mL @ 37°C
Unit Definition One unit will catalyze the transphosphorylation of one micromole of phosphate from creatine phosphate to ADP per minute at 37°C.
Formulation Solution in 20 mM potassium phosphate, 1 mM DTT, 50% glycerol (v/v), pH 5 - 6.
Appearance Clear, colorless solution
Additional Info Heterodimer comprised of recombinant, full-length B subunit (accession P12277, 2 – 381) and recombinant M subunit without the C-terminal lysine (accession P06732, 2 – 380)
Storage -70°C
Recertification 2 years
Application ELISA, Western Blot

Lee Biosolutions sells Recombinant Human Creatine Kinase MB (CK-MB) for ELISA and Western blot applications, among others.

Custom preparations, technical support, bulk quantities and aliquoting available, email for more details.

Recombinant full length Creatine Kinase MB isoenzyme without the C-terminal lysine on the M subunit. CK-MB is a dimeric protein comprised of M and B subunits. The doublet band migrating approximately 44 kDa is not resolved on most gels. Purified in the enzymatically active form and reacts with monoclonal antibodies specific to MB isoenzyme in ELISA.

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190-24-R1-0.1 $ 269.00 100 ug
190-24-R1-1 $ 1,029.00 1 mg