Isocitrate Dehydrogenase (IDH, NADP+)

Catalogue Number: 341-02
Description
Product Name Isocitrate Dehydrogenase
Source Porcine Heart
Catalogue Number 341-02
Form Liquid
Purity Partially Purified
Activity > 10 U/mL (Kinetic, spectrophotometric)
Unit Definition One unit will catalyze the oxidation of one micromole of isocitrate and the reduction of one micromole of NADP per minute at pH 7.4 and 37°C.
Protein > 10 mg/mL (Coomassie)
Specific Activity > 1 U/mg protein
Formulation Suspension in 3.3 M ammonium sulfate, 50 mM MES and 1 mM manganese sulfate, pH 6.4.
Appearance Brown suspension
Handling - Mix thoroughly before sampling
- Dilute with 100 mM triethanolamine chloride, 50 mM sodium chloride, 1% BSA, pH 8.0 or another suitable buffer
Recertification 2 years
Storage 2-8°C
Synonyms NADP(+)-ICDH, NADP, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate Decarboxylase
CAS Number 9028-48-2
E.C. Number 1.1.1.42
Gene IDH1, ICDH
Reaction Isocitrate + NADP(+) <=> 2-oxoglutarate + CO(2) + NADPH
Applications
Overview

Lee Biosolutions producer and supplier of Isocitrate Dehydrogenase-ICDH - NADP is from porcine heart for laboratory research and manufacturing clinical diagnostic assays.

ICDH is available in bulk and research quantities. Inquire on custom buffers and specifications.

Isocitrate dehydrogenase is an enzyme that participates in the citric acid cycle. ICDH catalyzes the third step of the cycle: the oxidative decarboxylation of isocitrate, producing alpha-ketoglutarate (a-ketoglutarate) and CO2 while converting NAD+ to NADH. This is a two-step process, which involves oxidation of isocitrate (a secondary alcohol) to oxalosuccinate (a ketone), followed by the decarboxylation of the carboxyl group beta to the ketone, forming alpha-ketoglutarate.

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SKU PRICE (USD) UNIT QTY
341-02-1 $ 310.00 1 kU
341-02-10 $ 1,292.00 10 kU