Product Name Alpha-Amylase
Source Human Saliva
Catalogue Number 120-10
Purity > 90% (SDS-PAGE)
Purity Note Typically > 98% (SDS-PAGE, two bands at approx. 60kDa)
Form Lyophilized
Activity > 100 U/mg (Siemens Dimension® Clinical Chemistry System)
Unit Definition One unit will catalyze the hydrolysis of one micromole 2-chloro-4-nitrophenyl-a-D-maltotrioside to yield 2-chloro-4-nitrophenol per minute at 37°C.
Protein > 0.2 mg protein/mg (Coomassie)
Specific Activity > 400 U/mg protein
Contaminants Lipase: < 0.1%
Protease: < 0.1%
Ammonia: < 0.1 micromole/mg
pH 6.0 - 7.5 (10 mg/mL H2O)
Formulation Lyophilized from Tris Chloride, Mannitol pH 7.2
Appearance White to Off-white powder
Solubility Clear, colorless (10 mg/mL saline)
Reconstitution Saline at ≥ 100 units/mL. Further dilutions should be in the lyophilization buffer (75 mM sodium chloride, 10 mM tris chloride, 1 mM calcium chloride, pH 7.2) or a similar solution. Calcium is important for the best activity and stability.
Related Products 120-17 - α-Amylase from human saliva - Liquid
400-20 - Lipase from human pancreas
122-40 - β-Amylase from sweet potato
Storage -20°C
Recertification 3 years
Infectious Disease Testing Negative or non-reactive at the donor level for anti-HIV 1 and 2, anti-HCV, HBsAg, HIV-1 (NAT), HBV (NAT), HCV (NAT), and syphilis by FDA approved methods.
Synonyms 1,4-alpha-D-Glucan-glucanohydrolase, ptyalin
Molecular Weight ~60,000
CAS Number 9000-90-2
E.C. Number
Gene AMY1A
Gene ID 276
Accession No P04745
References Journal of Biosciences, 2013, Volume 38, Number 2, Page 259