Glutamate Dehydrogenase (GLDH)

Product Name Glutamate Dehydrogenase
Source Bovine Liver
Catalogue Number 260-10
Purity Purified
Form Lyophilized
Activity > 15 U/mg
Unit Definition One unit catalyzes the oxidation of one micromole of NADH and the reductive amination of one micromole of alpha-ketoglutarate per minute at 37°C and pH 7.95 in the presence of ADP.
Protein > 0.25 mg protein/mg (Coomassie)
Specific Activity > 20 U/mg protein
Contaminants AST/SGOT: < 5%
ALT/GPT: < 5%
Ammonia: < 0.1 micromole/mg
Formulation Lyophilized from sodium citrate and mannitol buffer.
Appearance Tan powder
Recertification 3 years
Storage -20°C
Application Used for the determination of Urea in serum or plasma assays.
Function Glutamate dehydrogenase is used to catalyze the conversion of isocitrate into α-ketoglutarate and carbon dioxide.
Synonyms NAD(P)+, GDH1, L-GDH, GDH, Glutamate dehydrogenase 1, mitochondrial, L-Glutamate:NAD(P)+ oxidoreductase
Molecular Weight ~350,000
CAS Number 9029-12-3
E.C. Number 1.4.1.3
Gene GLUD1, BOS_24262, GDH
Gene ID 281785
Accession No P00366