Aspartate Aminotransferase (AST, GOT)

Product Name Aspartate Aminotransferase
Source Porcine Heart
Catalogue Number 300-20
Purity Purified
Form Lyophilized
Activity > 100 U/mg (Dimension® Clinical Chemistry System)
Unit Definition One unit will catalyze the transamination of one micromole of L-aspartate to alpha-ketoglutarate forming L-glutamate and oxaloacetate per minute at 37°C and pH 7.8
Protein > 0.7 mg protein/mg solid (A280)
Report mg protein/mg solid (Coomassie)
Specific Activity > 110 U/mg protein (A280)
Report U/mg protein (Coomassie)
Contaminants CPK: < 0.1%
GPT/ALT: < 0.1%
LDH: < 0.1%
GLDH: < 0.1%
MDH: < 0.01%
Protease: < 0.1%
Ammonia: < 0.005 micromole/mg
Formulation Lyophilized from sodium phosphate and pyridoxal-5-phosphate, pH 7.0.
Appearance Yellow to yellow-green powder
Reconstitution > 1 mg/mL in tris buffered saline, 1% BSA, pH 8.0.
Isoform Cytosolic
Related Products 480-20 - Alkaline Phosphatase from calf intestine
310-30 - Alanine Aminotransferase from porcine heart
350-50 - Lactate Dehydrogenase from chicken heart
Storage -20°C
Recertification 3 years
Application Control, Calibrator, Research, Manufacturing
Synonyms Aspartate Transaminase, glutamate oxaloacetate transaminase, AST, GOT, sGOT, AspAT, ASAT, AAT, serum glutamic oxaloacetic transaminase
Molecular Weight ~92,000 (Feliss and martinez-Carrion 1970)
CAS Number 9000-97-9
E.C. Number
Gene GOT1
Accession No P00503