Alkaline Phosphatase (CIP/CIAP)

Catalogue Number: 480-20
Product Name Alkaline Phosphatase
Source Calf Intestines
Catalogue Number 480-20
Purity Highly Purified
Form Lyophilized
Activity > 210 U/mg (Siemens Dimension® Clinical Chemistry System)
Unit Definition One unit will convert one micromole of p-nitrophenylphosphate to p-nitrophenol and phosphate per minute at 37°C in the presence of AMP (2-amino-2-methyl-1-propanol) at pH 10.35 and 37°C.
Protein > 0.4 mg protein/mg (Coomassie)
Specific Activity > 280 U/mg protein (Typically > 1,000 U/mg protein)
Contaminants Ammonia: < 1.0 micromole/mg
Formulation Lyophilized from tris-chloride and magnesium chloride, pH 8.3
Appearance Off-white to tan powder
Solubility Clear, colorless in 1 mg/mL saline.
Reconstitution Tris buffered saline with 1 mM magnesium chloride, 0.2 mM zinc chloride, and 1% BSA, pH 8.0.
Related Products 490-20 - Alkaline Phosphatase from porcine kidney
310-30 - Alanine Aminotransferase from porcine heart
300-20 - Aspartate Aminotransferase from porcine heart
Storage -20°C
Recertification 3 years
Molecular Weight Consists of two identical subunits of 65kDa
Synonyms CIP, CIAP, ALP, Orthophosphoric-Monoester Phosphohydrolase
CAS Number 9001-78-9
E.C. Number
Gene ALPI, BOS_2150, IAP
Gene ID 280993
References J Pediatr Gastroenterol Nutr. 2011 Oct; 53(4): 371–379.

Lee Biosolutions is the leading producer of Calf Intestine Alkaline Phosphatase (CIAP, ALP, ALKP) for research and bulk manufacturing for clinical diagnostics.

Custom preparations, technical support, bulk quantities and aliquoting available, email for more details.

Alkaline Phosphatase (ALP) is a hydrolase enzyme responsible for removing phosphate groups in the 5- and 3- positions from many types of molecules, including nucleotides, proteins, and alkaloids. In humans, alkaline phosphatase is present in all tissues throughout the entire body, but is particularly concentrated in liver, bile duct, kidney, bone, and the placenta.

Add to Cart
480-20-2.5 $ 91.00 2.5 kU
480-20-10 $ 266.00 10 kU
480-20-50 $ 1,132.00 50 kU