Alkaline Phosphatase (CIP/CIAP)

Catalogue Number: 480-20
Description
Product Name Alkaline Phosphatase
Source Calf Intestines
Catalogue Number 480-20
Purity Highly Purified
Form Lyophilized
Activity > 210 U/mg (Siemens Dimension® Clinical Chemistry System)
Protein > 0.4 mg protein/mg (Coomassie)
Specific Activity > 280 U/mg protein (Typically > 1,000 U/mg protein)
Unit Definition One unit will convert one micromole of p-nitrophenylphosphate to p-nitrophenol and phosphate per minute at 37°C in the presence of AMP (2-amino-2-methyl-1-propanol) at pH 10.35 and 37°C.
Contaminants Ammonia: < 1.0 micromole/mg
Form Lyophilized from tris-chloride and magnesium chloride, pH 8.3
Appearance Off-white to tan powder
Reconstitution Tris buffered saline with 1 mM magnesium chloride, 0.2 mM zinc chloride, and 1% BSA, pH 8.0.
Solubility Clear, colorless in 1 mg/mL saline.
Storage -20°C
Recertification 3 years
Molecular Weight Consists of two identical subunits of 65kDa
Synonyms CIP, CIAP, ALP, Orthophosphoric-Monoester Phosphohydrolase
CAS Number 9001-78-9
E.C. Number 3.1.3.1
Gene ALPI, BOS_2150, IAP
Gene ID 280993
Overview

Lee Biosolutions is the leading producer of Calf Intestine Alkaline Phosphatase (CIAP, ALP, ALKP) for research and bulk manufacturing for clinical diagnostics.

Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.

Alkaline Phosphatase (ALP) is a hydrolase enzyme responsible for removing phosphate groups in the 5- and 3- positions from many types of molecules, including nucleotides, proteins, and alkaloids. In humans, alkaline phosphatase is present in all tissues throughout the entire body, but is particularly concentrated in liver, bile duct, kidney, bone, and the placenta.

Order
SKU PRICE (USD) UNIT QTY
480-20-2.5 $ 60.00 2.5 kU
480-20-10 $ 175.00 10 kU
480-20-50 $ 750.00 50 kU