Alkaline Phosphatase (ALP)

Catalogue Number: 490-20
Description
Product Name Alkaline Phosphatase
Source Porcine Kidney
Catalogue Number 490-20
Purity Purified
Form Lyophilized
Activity > 50 U/mg (Dimension® Clinical Chemistry System)
Protein > 0.5 mg protein/mg solid (Coomassie)
Specific Activity > 50 U/mg protein
Contaminants gGT: < 2%
AST/SGOT: < 1.0%
LDH: < 0.1%
Ammonia: < 0.1 micromole/mg
Unit Definition One unit catalyzes the hydrolysis of one micromole of p-nitrophenyl phosphate per minute at 37°C and pH 10.35 in the presence of 2-amino-2-methyl-1-propanol.
Appearance Tan powder
Solubility Clear, tan solution in 1 mg/mL saline.
Form Lyophilized from tris chloride with magnesium chloride and zinc chloride, pH 8.0.
Recertification 3 years
Storage -20°C
Synonyms ALP, Orthophosphoric-Monoester Phosphohydrolase
CAS Number 9001-78-9
E.C. Number 3.1.3.1
Overview

Lee Biosolutions is the leading producer of porcine kidney Alkaline Phosphatase (ALP) for research, life science and diagnostic manufacturing.

Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.

Alkaline Phosphatase is a hydrolase enzyme responsible for removing phosphate groups in the 5- and 3- positions from many types of molecules, including nucleotides, proteins, and alkaloids. In humans, alkaline phosphatase is present in all tissues throughout the entire body, but is particularly concentrated in liver, bile duct, kidney, bone, and the placenta. The optimal pH for the enzyme activity is 10.0 in standard conditions.

Order
SKU PRICE (USD) UNIT QTY
490-20-1 $ 60.00 1 kU
490-20-5 $ 195.00 5 kU
490-20-25 $ 750.00 25 kU