Aspartate Aminotransferase (AST, GOT)
|Product Name||Aspartate Aminotransferase (AST, GOT)|
|Activity||> 100 U/mg (Dimension® Clinical Chemistry System)|
|Protein||> 0.7 mg protein/mg solid (A280)
Report mg protein/mg solid (Coomassie)
|Specific Activity||> 110 U/mg protein (A280)|
|Unit Definition||One unit will catalyze the transamination of one micromole of L-aspartate to alpha-ketoglutarate forming L-glutamate and oxaloacetate per minute at 37°C and pH 7.8|
|Contaminants||CPK: < 0.1%
GPT/ALT: < 0.1%
LDH: < 0.1%
GLDH: < 0.1%
MDH: < 0.1%
Protease: < 0.01%
Ammonia: < 0.01 micromole/mg
|Form||Lyophilized from sodium phosphate and pyridoxal-5-phosphate, pH 7.0.|
|Reconstitution||> 1 mg/mL in tris buffered saline, 1% BSA, pH 8.0.|
|Appearance||Typically yellow-green powder|
|Application||Control, Calibrator, Research, Manufacturing|
|Synonyms||Aspartate Transaminase, glutamate oxaloacetate transaminase, AST, GOT, sGOT, AspAT, ASAT, AAT, serum glutamic oxaloacetic transaminase|
|Molecular Weight||~92,000 (Feliss and martinez-Carrion 1970)|
Lee Biosolutions produces Porcine Heart Aspartate Transferase (AST), also known as Glutamate Oxaloacetate Transaminase (GOT) for research and diagnostic manufacturing uses.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details. Single batch sizes from 100,000 kU.
There are two SGOT/AST isoenzymes are present in humans, they have high similarity. GOT1, the cytosolic isoenzyme derives mainly from red blood cells and heart. GOT2, the mitochondrial isoenzyme is predominantly present in liver.