Aspartate Aminotransferase (AST, GOT)

Catalogue Number: 300-20
Description
Product Name Aspartate Aminotransferase
Source Porcine Heart
Catalogue Number 300-20
Purity Purified
Form Lyophilized
Activity > 100 U/mg (Dimension® Clinical Chemistry System)
Unit Definition One unit will catalyze the transamination of one micromole of L-aspartate to alpha-ketoglutarate forming L-glutamate and oxaloacetate per minute at 37°C and pH 7.8
Protein > 0.7 mg protein/mg solid (A280)
Report mg protein/mg solid (Coomassie)
Specific Activity > 110 U/mg protein (A280)
Report U/mg protein (Coomassie)
Contaminants CPK: < 0.1%
GPT/ALT: < 0.1%
LDH: < 0.1%
GLDH: < 0.1%
MDH: < 0.01%
Protease: < 0.1%
Ammonia: < 0.005 micromole/mg
Formulation Lyophilized from sodium phosphate and pyridoxal-5-phosphate, pH 7.0.
Appearance Yellow to yellow-green powder
Reconstitution > 1 mg/mL in tris buffered saline, 1% BSA, pH 8.0.
Isoform Cytosolic
Related Products 480-20 - Alkaline Phosphatase from calf intestine
310-30 - Alanine Aminotransferase from porcine heart
350-50 - Lactate Dehydrogenase from chicken heart
Storage -20°C
Recertification 3 years
Application Control, Calibrator, Research, Manufacturing
Synonyms Aspartate Transaminase, glutamate oxaloacetate transaminase, AST, GOT, sGOT, AspAT, ASAT, AAT, serum glutamic oxaloacetic transaminase
Molecular Weight ~92,000 (Feliss and martinez-Carrion 1970)
CAS Number 9000-97-9
E.C. Number 2.6.1.1
Gene GOT1
Accession No P00503
Overview

Lee Biosolutions produces Porcine Heart Aspartate Transferase (AST), also known as Glutamate Oxaloacetate Transaminase (GOT) for research and diagnostic manufacturing uses.

Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details. Single batch sizes from 100,000 kU.

There are two SGOT/AST isoenzymes are present in humans, they have high similarity. GOT1, the cytosolic isoenzyme derives mainly from red blood cells and heart. GOT2, the mitochondrial isoenzyme is predominantly present in liver.

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SKU PRICE (USD) UNIT QTY
300-20-5 $ 53.00 5 kU
300-20-25 $ 171.00 25 kU
300-20-100 $ 526.00 100 kU
300-20-250 Inquire 250 kU