Aspartate Aminotransferase (AST, GOT)

Catalogue Number: 300-20
Product Name Aspartate Aminotransferase (AST, GOT)
Source Porcine Heart
Catalogue Number 300-20
Purity Purified
Form Lyophilized
Activity > 100 U/mg (Dimension® Clinical Chemistry System)
Protein > 0.7 mg protein/mg solid (A280)
Report mg protein/mg solid (Coomassie)
Specific Activity > 110 U/mg protein (A280)
Report U/mg protein (Coomassie)
Unit Definition One unit will catalyze the transamination of one micromole of L-aspartate to alpha-ketoglutarate forming L-glutamate and oxaloacetate per minute at 37°C and pH 7.8
Contaminants CPK: < 0.1%
GPT/ALT: < 0.1%
LDH: < 0.1%
GLDH: < 0.1%
MDH: < 0.01%
Protease: < 0.1%
Ammonia: < 0.005 micromole/mg
Form Lyophilized from sodium phosphate and pyridoxal-5-phosphate, pH 7.0.
Reconstitution > 1 mg/mL in tris buffered saline, 1% BSA, pH 8.0.
Appearance Typically yellow-green powder
Isoform Cytosolic
Storage -20°C
Recertification 3 years
Application Control, Calibrator, Research, Manufacturing
Synonyms Aspartate Transaminase, glutamate oxaloacetate transaminase, AST, GOT, sGOT, AspAT, ASAT, AAT, serum glutamic oxaloacetic transaminase
Molecular Weight ~92,000 (Feliss and martinez-Carrion 1970)
CAS Number 9000-97-9
E.C. Number
Gene GOT1
Accession No P00503

Lee Biosolutions produces Porcine Heart Aspartate Transferase (AST), also known as Glutamate Oxaloacetate Transaminase (GOT) for research and diagnostic manufacturing uses.

Custom preparations, technical support, bulk quantities and aliquoting available, email for more details. Single batch sizes from 100,000 kU.

There are two SGOT/AST isoenzymes are present in humans, they have high similarity. GOT1, the cytosolic isoenzyme derives mainly from red blood cells and heart. GOT2, the mitochondrial isoenzyme is predominantly present in liver.

300-20-5 $ 50.00 5 kU
300-20-20 $ 130.00 20 kU
300-20-50 $ 250.00 50 kU
300-20-250 $ 1,200.00 250 kU