Leukocytes, Sonicated

Product Name	Sonicated Leukocytes
Source	Human Leukocytes
Catalogue Number	342-10
Form	Liquid
Purity	Blood Fraction
Activity	5.0 - 6.0 U/mL (Elastase)
Unit Definition	One unit will catalyze the hydrolysis of one micromole of methoxy succinyl-alanine-proline-valine-p-nitroanilide per minute at 37°C and pH 7.5 (In the presence of 500 mM sodium chloride and 0.2% Triton X-100)
Protein	> 1.0 mg/mL (Coomassie)
Dipstick Assay	Leukocytes: > 2+ Protein: Negative Glucose: Negative Blood: Less than trace
Dipstick Protocol	0.9 mL per liter of saline will give a positive reaction for leukocytes with Roche Chemstrip^® 10 SG and/or Siemens Multistix^® 10 SG test strips.
Formulation	Suspension in 154 mM sodium chloride.
Appearance	Milky white suspension. May form aggregates due to freeze/thaws.
Handling	Sonicate prior to use to disperse aggregates.
Related Products	910-12 - Bilirubin Conjugate, Ditaurate, Sodium Salt 341-10 - Whole Leukocytes from human 343-10 - Esterase from porcine liver
Storage	-20°C
Recertification	2 years
Infectious Disease Testing	Negative or non-reactive at the donor level for anti-HIV 1 and 2, anti-HCV, HBsAg, HIV-1 (NAT), HBV (NAT), HCV (NAT), and syphilis by FDA approved methods.
Synonyms	Immune cells, LE, Elastase