Esterase (PLE)

Catalogue Number: 343-10
Description
Product Name Esterase
Source Porcine Liver
Catalogue Number 343-10
Purity Purified
Form Lyophilized
Activity > 75 U/mg powder
Unit Definition One unit will catalyze the hydrolysis of one micromole of ethyl butyrate to butyrate and ethanol per minute at pH 8.0 and 37°C
Protein > 0.4 mg protein/mg (Coomassie)
Specific Activity Reported in U/mg protein
Formulation Lyophilized from sodium phosphate, pH 7.5.
Ammonia < 0.01 μmole/mg
Related Products 127-12 - Bilirubin, Unconjugated - Indirect
651-10 - Urobilinogen
188-10 - Cholesterol Esterase from porcine pancreas
Storage -20°C
Recertification 3 years
Synonyms Carboxylesterase, Carboxyl esterase, Carboxylic-ester hydrolase, PLE
Application Asymmetric Ester Hydrolysis, Research, Life Science, Manufacturing, Urinalysis
Raw Material Source United States of America (USDA licensed establishment)
Molecular Weight 162,000
CAS Number 9016-18-6
E.C. Number 3.1.1.1
Gene CES1, APLE, PMPMEase, REH, TGH
Gene ID 397478
References 1) A rapid oxygen exchange on prostaglandins in plasma represents plasma esterase activity that is inhibited by diethylumbelliferyl phosphate with high affinity, Rapid Communications in Mass Spectrometry, Volume 26, Issue 20, 30 October 2012, Pages 2472-2476.
Overview

Lee Biosolutions is the largest producer of Esterase from porcine liver used in urine controls and for kinetic resolutions and asymmetric ester hydrolysis in organic chemistry.

Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details. 250,000 kU single lot sizes.

Liver esterases are heterogeneous enzymes responsible for detoxification of xenobiotics and activation of ester and amide prodrugs. They catalyze the hydrolysis of a carboxylic esters to the corresponding alcohols and carboxylic acids. These enzymes have wide substrate specificities[i], are resistant to denaturation[ii], and retain activity under near-anhydrous conditions[iii].

Lee Biosolutions manufactures esterase from porcine (Sus scrofa, domestic pig) liver in bulk quantities for use in synthesis, preparation of clinical controls, and research.


Identification

1.Synonyms: PLE, Liver carboxylesterase, Proline-beta-naphthylamidase, Retinyl ester hydrolase

2.EC #: 3.1.1.1

3.CAS #: 9016-18-6

4.Accession #: Q29550, EST_1PIG (19 – 566)


Properties

1.Molecular function: carboxylic ester hydrolase

Carboxylic ester + Water = Alcohol + Carboxylic acid

2.Inhibitors: diisopropylflurophosphate, phenylmethylsulfonyl fluoride[iv], tosyl-L-phenylalanine chloromethyl ketone[v]

3.Activator: CHAPS[vi]

4.Stability: Stable at neutral pH, frozen or lyophilized. Loses activity rapidly at pH 4.5 and belowvii

5.pH optimum: 6 – 8i,iv

6.Structure: 60,074 MW monomervi (sequence), 162,000 polymerizes readily at neutral pHvii

7.Post-translational modifications

a.Residue 80: N-linked (GlcNAc…) asparagine

b.Disulfide bonds (88 – 117 and 274 – 285)

c.Residue 379: Phosphoserine

8.Isoelectric point: 5.0vii

9.Extinction coefficient (E0.1%280 nm): 1.3[vii]

Diagnostic Relevance: Surrogate for preparation of leukocyte esterase controls or calibrators in urinalysis. Urinary leukocyte esterase is an indicator of urinary tract infection.

Other Applications: Enantioselective biocatalysis[viii],[ix],[x]


[i] www.brenda-enzymes.org

[ii] Farb, D. and Jencks, W.P., Arch. Biochem. Biophys. 203, 214-226 (1980)

[iii] Lopez, M. et al., Biophys. J. 99, L62-L64 (2010)

[iv] Junge, W. and Heymann, E., Eur. J. Biochem. 95, 519-525 (1979)

[v] Oboh, O.T. and Lamango, N.S., J. Biochem. Mol. Toxicol. 22, 51-62 (2008)

[vi] www.uniprot.org

[vii] Dudman, N.P.B and Zerner, B., Meth. Enzymol. 24 190-208 (1970)

[viii] Henke, E., et al., ChemBioChem 4, 485-493 (2003)

[ix] Sousa, H.A. et al., J. Chem. Technol. Biotechnol. 75, 707-714 (2000)

[x] Tamm, C., Pure & Appl. Chem. 64, 1187-1191 (1992)

Add to Cart
SKU PRICE (USD) UNIT QTY
343-10-5 $ 53.00 5 kU
343-10-25 $ 205.00 25 kU
343-10-100 $ 420.00 100 kU
343-10-1,000 Inquire 1,000 kU