|Protein||25 g/L (UBG assay)|
|Appearance||Amber to orange liquid|
|pH||13.2 +/- 0.2 (20°C)|
|Storage||-20°C - Protect from air and light - Store under argon|
|Molecular Weight||590.72 - 598.78 g/mol|
Lee Biosolutions sells Synthetic Urobilinogen for use as an indicator in urine controls and test strips and research purposes.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
Urine Test Strip: Modified Ehrlich's reaction. Urobilinogen present reacts with Ehrlich's reagent to form a red-colored compound. Color changes from light orange-pink to dark pink. The test is based on a diazotisation reaction of 4- Methoxybenzene diazoniurn salt and urinary urobilinogen in a strong acid medium. The color changes from pink to brown-red.
Urobilinogen is a chromogen formed in the intestine from the breakdown of a reddish-yellow water insoluble pigment.
Urobilinogen is a bile pigment that is produced by the degradation of conjugated bilirubin in the biliary tree and small intestine. It is water soluble, colorless, and has several fates: partial oxidation to urobilin partial re-absoption in the small intestine and re-circulation back to the liver - enterohepatic circulation re-absorption into blood and passage to kidney for excretion.
Urobilinogen is present in the urine of normal subjects. If there is an increase in urobilinogen in the urine then this indicates hepato-cellular dysfunction or an increased bilirubin formation, e.g. due to haemolysis. In intrahepatic obstruction there is no urobilinogen in the urine.