Lactate Dehydrogenase (LDH)

Product Name Lactate Dehydrogenase
Source Bovine Heart
Catalogue Number 350-11
Purity Purified
Form Liquid
Activity 2,000 - 2,400 U/mL (Siemens Dimension® Clinical Chemistry System)
Unit Definition One unit will catalyze the oxidation of one micromole of L-lactate to pyruvate with simultaneous reduction of NAD+ to NADH per minute at 37°C and pH 9.4.
Protein 5 - 20 mg protein/mL (Coomassie)
Specific Activity > 100 U/mg protein
Contaminants AST/GOT: < 0.6%
ALT/GPT: < 0.07%
Formulation Suspension in 3.1 M ammonium sulfate
Appearance White to off-white slurry
Recertification 2 years
Storage 2-8°C
Reaction Catalyzes the conversion of pyruvate to lactate and back, as it converts NADH to NAD+ and back.
Synonyms LAD, LD, L-LDH, (S)-Lactate: NAD+ oxidoreductase
Molecular Weight Appr. 136,700 (Huston et al. 1972)
CAS Number 9001-60-9
E.C. Number 1.1.1.27