Alpha-Amylase

Catalogue Number: 120-10
Description
Product Name Alpha-Amylase
Source Human Saliva
Catalogue Number 120-10
Purity > 90% (SDS-PAGE)
Purity Note Typically > 98% (SDS-PAGE, two bands at approx. 60kDa)
Form Lyophilized
Activity > 100 U/mg (Siemens Dimension® Clinical Chemistry System)
Unit Definition One unit will catalyze the hydrolysis of one micromole 2-chloro-4-nitrophenyl-a-D-maltotrioside to yield 2-chloro-4-nitrophenol per minute at 37°C.
Protein > 0.2 mg protein/mg (Coomassie)
Specific Activity > 400 U/mg protein
Contaminants Lipase: < 0.1%
Protease: < 0.1%
Ammonia: < 0.1 micromole/mg
pH 6.0 - 7.5 (10 mg/mL H2O)
Formulation Lyophilized from 75 mM sodium chloride, 10 mM Tris, 1 mM calcium chloride, pH 7.2.
Appearance Off-white powder
Solubility Clear, colorless (1 mg/mL saline)
Reconstitution Saline at ≥ 100 units/mL. Further dilutions should be in the lyophilization buffer (75 mM sodium chloride, 10 mM tris chloride, 1 mM calcium chloride, pH 7.2) or a similar solution. Calcium is important for the best activity and stability.
Related Products 120-17 - α-Amylase from human saliva - Liquid
400-20 - Lipase from human pancreas
122-40 - β-Amylase from sweet potato
Storage -20°C
Recertification 3 years
Infectious Disease Testing Negative or non-reactive at the donor level for anti-HIV 1 and 2, anti-HCV, HBsAg, HIV-1 (NAT), HBV (NAT), HCV (NAT), and syphilis by FDA approved methods.
Synonyms 1,4-alpha-D-Glucan-glucanohydrolase, ptyalin
Molecular Weight ~60,000
CAS Number 9000-90-2
E.C. Number 3.2.1.1
Gene AMY1A
Gene ID 276
Accession No P04745
References Journal of Biosciences, 2013, Volume 38, Number 2, Page 259
Overview

Lee Biosolutions produces and manufactures high purity human salivary Amylase for research and diagnostic manufacturing uses.

Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.

Amylase is an enzyme that breaks starch down into sugar. Amylase is present in human saliva, where it begins the chemical process of digestion. Foods that contain much starch but little sugar, such as rice and potato, taste slightly sweet as they are chewed because amylase turns some of their starch into sugar in the mouth.

Alpha-Amylase is an enzyme that hydrolyses alpha-bonds of large alpha-linked polysaccharides such as starch and glycogen, yielding glucose and maltose. It is being used by researchers as a barometer of the body's response to physical or psychological stress. NIH study shows that Women with high levels of alpha amylase, indicating stress, are less likely to conceive.

Add to Cart
SKU PRICE (USD) UNIT QTY
120-10-1 $ 58.00 1 kU
120-10-10 $ 368.00 10 kU
120-10-25 $ 866.00 25 kU
Representative SDS-PAGE - 120-10