Pyruvate Kinase (PK)

Catalogue Number: 500-20
Description
Product Name Pyruvate Kinase
Source Rabbit Muscle
Catalogue Number 500-20
Purity Purified
Form Lyophilized
Activity > 200 U/mg
Unit Definition One unit will catalyze the conversion of 1.0 micromole of phosphoenolpyruvate to pyruvate per minute at pH 7.6 at 37°C
Protein > 0.4 mg protein/mg solid (A280nm, E0.1%=0.54)
Specific Activity Report U/mg protein
Contaminants CPK: < 1.0%
LDH: < 0.01%
Appearance White to off-white powder
Formulation Powder, lyophilized from tris chloride, pH 7.7.
Related Products 490-76 - PGM from rabbit muscle
191-50 - CPK from rabbit muscle
276-50 - G3PDH from rabbit muscle
Storage -20°C
Recertification 3 years
Synonyms ATP:Pyruvate 2-0-phosphotransferase, PK
Action Pyruvate kinase catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP.
This transfer yields one molecule of pyruvate and one molecule of ATP.
Application Enzymatic determination of ADP, ATP, pyruvate or phsphoenolpyruvate (PEP) when coupled with the related enzymes.
Molecular Weight 237 kDa (as a tetramer of four equal subunits 57 kDa)
Reaction ATP + pyruvate = ADP + phosphoenolpyruvate
E.C. Number 2.7.1.40
CAS Number 9001-59-6
Gene PKM, PKM2
Gene ID 100008676
Accession No P14618
References Vascular Pharmacology, Volume 59, Issues 3–4, September–October 2013, Pages 76-82
Applications
Overview

Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.

Pyruvate Kinase is an enzyme involved in glycolysis. Pyruvate Kinase (PK) catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding a pyruvate molecule and producing one molecule ATP.

Add to Cart
SKU PRICE (USD) UNIT QTY
500-20-25 $ 93.00 25 kU
500-20-100 $ 308.00 100 kU
500-20-500 $ 1,322.00 500 kU