Pregnant Mare Serum Gonadotropin (PMSG)

Catalogue Number: 493-10
Product Name Pregnant Mare Serum Gonadotropin
Source Equine Serum
Catalogue Number 493-10
Form Lyophilized
Purity Highly Purified
Activity > 1,000 IU/mg powder
Additional Info Luteinizing hormone-like activity
Appearance White to off-white solid
Recertification 2 years
Storage 2-8°C
Reconstitution 1 mg/mL is deionized water. Further dilutions should be in a buffer such as tris buffered saline with 1% BSA as a carrier.
Synonyms PMSG, eCG, Equine Chorionic Gonadotropin, Anteron; Antostab; Eleagol; Equine Cyonin; Equine Gonadotropin; Seragon; Serotropin; Predalon-S; Priatin, Gonadyl1
CAS Number 9002-70-4
E.C. Number 232-663-9
Accession No P01220 (alpha subunit, 25 – 120), P08751 (beta subunit, 21 – 169)

Custom preparations, technical support, bulk quantities and aliquoting available, email for more details.

Pregnant mare serum gonadotropin (PMSG) is a glycoprotein hormone synthesized in the domestic horse placenta. Commercial preparations are isolated from the serum of pregnant mares. The hormone is a dimer of non-covalently associated alpha and beta subunits. It is a unique member of the gonadotropin family in that it contains high levels of FSH and LH activity[i]. Its ability to stimulate ovulation is determined in a bioassay, and the results are expressed as international units (IU)[ii]. PMSG that has been purified to homogeneity has approximately 16,000 IU/mg protein[iii]. PMSG may be used to induce ovulation in livestock prior to artificial insemination.

[i] Moore, W.T., Jr. and D.N. Ward, JBC 255, 6923 (1980)

[ii] Cole, H.H. and J. Erway, Endocrinology 29, 514 (1941)

[iii] Gospodarowicz, D. and H. Papkoff, Endocrinology 80, 699 (1967)

Research Applications

1.Induction of ovulation in experimental animals

2.Study of molecular determinants of LH and FSH activities

Properties (Equus caballus, horse)

1.Molecular function: hormone activity, expressed in IU

2.Structure: 64,030 MW heterodimer[i]

a.Alpha chain: 43,720 MW

b.Beta chain: 16,960 MW

3.Post-translational modification:

a.Alpha chain N-linked (GlcNAc…) asparagine, approximately 21% of mass, interchain disulfides

b.Beta chain O-linked (GalNAc…) serine and threonine, N-linked (GlcNAc…) asparagine, approximately 46% of mass, intrachain disulfides

4.Beta E0.1% = 0.238 (sequence)

[i] Christakos, S. and O.P. Bahl, JBC 254, 4253 (1979)

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493-10-2.5 $ 276.00 2,500 IU
493-10-10 $ 546.00 10,000 IU