Thrombin

Catalogue Number: 528-70
Description
Product Name Thrombin
Source Human Plasma
Catalogue Number 528-70
Purity > 95% (SDS-PAGE)
Form Lyophilized
Specific Activity > 2,800 NIH U/mg protein
Protein Report mg protein/mg solid or mg protein/vial (A280, E0.1% = 1.83)
Activity Report NIH U/mg solid or NIH U/vial
Formulation Lyophilized from 20 mM bis-tris, 150 mM sodium chloride, 0.1% PEG 8000, pH 6.5.
Solubility 1 mg/mL saline (Clear, colorless to light yellow solution)
Reconstitution - A working stock solution is prepared by adding 1 mL buffered saline, pH 7.4 (ex. 25mM HEPES, 150mM sodium chloride, 0.1% PEG 8000, pH 7.4).
- Further dilution should be made in buffer containing a suitable blocking agent such as 0.1%-1% Prionex, BSA or PEG.
Related Products 494-21 - Prothrombin from human plasma
271-17 - Fibrinogen from human plasma
200-13 - D-Dimer from human plasma
Storage 2-8°C
Recertification 3 years
Molecular Weight 36,000
Synonyms Factor IIa
UNSPSC code 51131907
CAS Number 9002-04-4
E.C. Number 3.4.21.5
Overview

Lee Biosolutions manufactures high purity human plasma Thrombin, in bulk and research quantities for diagnostic manufacturing.

Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, lot K. The NIH assay procedure used 0.2 mL plasma diluted 1:1 with saline as substrate and 0.1 mL of thrombin sample diluted in 1% buffered albumin solution. Based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

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SKU PRICE (USD) UNIT QTY
528-70-250 $ 138.00 250 units
528-70-1,000 $ 414.00 1,000 units
528-70-5,000 $ 1,379.00 5 x 1,000 units