Catalogue Number: 528-70
Product Name Thrombin
Source Human Plasma
Catalogue Number 528-70
Purity > 95% (SDS-PAGE)
Form Lyophilized
Specific Activity > 2,800 NIH U/mg protein
Protein Report mg protein/mg solid or mg protein/vial (A280, E0.1% = 1.83)
Activity Report NIH U/mg solid or NIH U/vial
Formulation Lyophilized from 20 mM bis-tris, 150 mM sodium chloride, 0.1% PEG 8000, pH 6.5.
Solubility 1 mg/mL saline (Clear, colorless to light yellow solution)
Reconstitution - A working stock solution is prepared by adding 1 mL buffered saline, pH 7.4 (ex. 25mM HEPES, 150mM sodium chloride, 0.1% PEG 8000, pH 7.4).
- Further dilution should be made in buffer containing a suitable blocking agent such as 0.1%-1% Prionex, BSA or PEG.
Related Products 494-21 - Prothrombin from human plasma
271-17 - Fibrinogen from human plasma
200-13 - D-Dimer from human plasma
Storage 2-8°C
Recertification 3 years
Molecular Weight 36,000
Synonyms Factor IIa
UNSPSC code 51131907
CAS Number 9002-04-4
E.C. Number

Lee Biosolutions manufactures high purity human plasma Thrombin, in bulk and research quantities for diagnostic manufacturing.

Custom preparations, technical support, bulk quantities and aliquoting available, email for more details.

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, lot K. The NIH assay procedure used 0.2 mL plasma diluted 1:1 with saline as substrate and 0.1 mL of thrombin sample diluted in 1% buffered albumin solution. Based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Add to Cart
528-70-250 $ 188.00 250 units
528-70-1,000 $ 565.00 1,000 units
528-70-5,000 $ 1,877.00 5 x 1,000 units