Thrombin
Catalogue Number: 528-70
Description
| Product Name | Thrombin |
| Source | Human Plasma |
| Catalogue Number | 528-70 |
| Purity | > 95% (SDS-PAGE) |
| Form | Lyophilized |
| Specific Activity | > 2,800 NIH U/mg protein |
| Protein | Report mg protein/mg solid or mg protein/vial (A280, E0.1% = 1.83) |
| Activity | Report NIH U/mg solid or NIH U/vial |
| Formulation | Lyophilized from 20 mM bis-tris, 150 mM sodium chloride, 0.1% PEG 8000, pH 6.5. |
| Solubility | 1 mg/mL saline (Clear, colorless to light yellow solution) |
| Reconstitution | - A working stock solution is prepared by adding 1 mL buffered saline, pH 7.4 (ex. 25mM HEPES, 150mM sodium chloride, 0.1% PEG 8000, pH 7.4). - Further dilution should be made in buffer containing a suitable blocking agent such as 0.1%-1% Prionex, BSA or PEG. |
| Related Products | 494-21 - Prothrombin from human plasma 271-17 - Fibrinogen from human plasma 200-13 - D-Dimer from human plasma |
| Storage | 2-8°C |
| Recertification | 3 years |
| Molecular Weight | 36,000 |
| Synonyms | Factor IIa |
| UNSPSC code | 51131907 |
| CAS Number | 9002-04-4 |
| E.C. Number | 3.4.21.5 |
Applications
Overview
Lee Biosolutions manufactures high purity human plasma Thrombin, in bulk and research quantities for diagnostic manufacturing.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, lot K. The NIH assay procedure used 0.2 mL plasma diluted 1:1 with saline as substrate and 0.1 mL of thrombin sample diluted in 1% buffered albumin solution. Based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
Add to Cart
Lee Biosolutions, Inc
