Thrombin

Catalogue Number: 528-50H
Description
Product Name Thrombin
Source Bovine Plasma
Catalogue Number 528-50H
Purity Highly Purified
Form Lyophilized
Activity > 200 U/mg solid (US/NIH coagulation units)
Protein Reported mg protein/mg solid (Biuret)
Specific Activity > 2,200 U/mg protein
Moisture Content < 5% (Loss on Drying)
pH 6.5 - 7.5 (1% solution)
Formulation Lyophilized powder containing glycine and sodium chloride.
Reconstitution Solubilize and dilute with 0.9% sodium chloride.
Storage -20°C
Recertification 3 years
Molecular Weight 36,000
Synonyms fibrinogenase, thrombase, thrombofort, topical, thrombin-C, tropostasin, activated blood-coagulation factor II, blood-coagulation factor IIa, factor IIa, E thrombin, beta-thrombin, gamma-thrombin
Country of Origin United States of America
CAS Number 9002-04-4
E.C. Number 3.4.21.5
Overview

Lee Biosolutions produces and supplies high purity bovine Thrombin for medical research of proteolytic enzymes and manufacturing of defibrinating human plasma and serum free media component.

Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.

Lee Biosolutions High Specific Activity Bovine Thrombin product is used to cleave r-fusion proteins containing a thrombin site for removal of affinity tags as well as certain diagnostic pharmaceutical applications.

Thrombin is a coagulation protein that has many effects in the coagulation cascade and is a serine protease that converts fibrinogen into fibrin. Bovine Thrombin used as a reagent, which has proven useful in the laboratory evaluation of many fibrinogens.

Our customers use Thrombin to defibrinate human plasma, producing serum matrices to be used as controls and standards around the world. Thrombin promotes chemotaxis in monocytes, neurite retraction in neuron Thrombin serves as a potent mitogenic agent in fibroblast growth and functions as a serum-free media component for several cell lines.

Bovine Thrombin is a multi-functional proteolytic enzyme which acts both specifically and non-specifically with several proteins, cells, surfaces, and other molecules. Bovine Thrombin has an essential role in the clotting process and has been well characterized where it converts fibrinogen, a soluble plasma glycoprotein, to an insoluble protein gel called fibrin, the essential component of the blood clot.

Bovine Thrombin is a clotting enzyme that is a serine protease which cleaves Arginine-Glycine bonds in fibrinogen to give clots of fibrin & fibrinpeptide A and B. Bovine Thrombin is derived from bovine prothrombin which is isolated from bovine plasma. Bovine prothrombin is treated with bovine lung thromboplastin to yield Bovine Thrombin and is also used to remove GST tags from proteins in purification process.

Add to Cart
SKU PRICE (USD) UNIT QTY
528-50H-2 $ 61.00 2,000 Units
528-50H-10 $ 166.00 10,000 Units
528-50H-50 $ 408.00 50,000 Units
528-50H-100 $ 607.00 100,000 Units