Apolipoprotein A1 (Apo A1)

Catalogue Number: 125-12
Product Name Apolipoprotein A1
Source Human Plasma
Catalogue Number 125-12
Purity > 90% (SDS-PAGE)
Form Liquid
Protein > 0.5 mg/mL (BCA vs. BSA standard)
Appearance Clear solution
Solubility Soluble in aqueous buffers at < 50 μg/mL.
Self-associates into oligomers at high concentrations in the absence of chaotropes (urea, guanidine hydrochloride).
Formulation Frozen, 0.2 micron filtered solution in 50 mM tris, 150 mM sodium chloride, 0.1% sodium azide, and EDTA, pH 7.4.
Storage -20°C
Recertification 2 years
Synonyms ApoA1, APO A1
Molecular Weight 28,300

Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.

Apo-AI comprises ~70% of the protein moiety in HDL. It is a single polypeptide chain consisting of 245 amino acids with glutamic acid as the C-terminal residue and aspartic acid as the N-terminal residue. The molecular mass is reported to be 28.3 kDa. The protein is made up of one major isoform (pI 5.6) and two minor isoforms (pI 5.53 and 5.46). Apo-AI shows a high content of a-helix structure.

The amphipathic regions in the á-helix structure seem to be responsible for lipid binding capacity. In aqueous solution, Apo-AI shows self-association with minor conformation change. Apo-AI activates lecithin-cholesterol (LCAT) acyltransferase, which is responsible for cholesterol esterification in plasma.

Apo-AI levels in normal plasma are 90-130 mg/dL. Apo-AI levels may be inversely related to the risk of coronary disease.

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125-12-0.1 $ 68.00 100 ug
125-12-1 $ 336.00 1 mg
125-12-10 Inquire 10 mg