Butyrylcholinesterase (BChE)

Product Name Butyrylcholinesterase
Source Equine Serum
Catalogue Number 130-10
Purity Highly Purified
Form Lyophilized
Activity > 50 U/mg (Siemens Dimension® Clinical Chemistry System)
Unit Definition A change of 0.2 milliabsorbance units (mA) per minute corresponds to pseudocholinesterase activity of 1 U/mL at 37 ̊C.
Protein > 0.1 mg protein/mg (A280nm, E = 1.36)
Specific Activity > 300 U/mg protein (Typically > 900 U/mg protein)
Appearance White to pale, grey-green powder
Reconstitution Reconstitute at 5 mg/mL using 50 mM Tris-HCl pH 7.3 - 7.5.
Solubility Clear, colorless to slightly green solution (1 mg/mL in 0.9% saline)
Related Products 135-10 - BCHE from human serum
343-10 - Esterase from porcine liver
Storage 2-8°C
Recertification 4 years
Synonyms BCHE, BuChE, PCHE, pseudocholinesterase, plasma cholinesterase, Acylcholine acyl-hydrolase, Choline esterase, butyryl
Application Diagnostic Manufacturing, Clinical Research
CAS Number 9001-08-5
E.C. Number 3.1.1.8
Gene BCHE
Accession No P06276 (human, 29 – 602), Q9N1N9 (equine)
Reaction Acylcholine + H2O = choline + carboxylic acid
Kinetics KM=18 µM (butyrylthiocholine, 25 degrees Celsius)
Activators Ca2+
Inhibitors Hg2+, EDTA, diisopropylfluorophosphate
Optimum pH 7 - 8