Butyrylcholinesterase (BChE)

Product Name	Butyrylcholinesterase
Source	Equine Serum
Catalogue Number	130-10
Purity	Highly Purified
Form	Lyophilized
Activity	> 50 U/mg (Siemens Dimension® Clinical Chemistry System)
Unit Definition	A change of 0.2 milliabsorbance units (mA) per minute corresponds to pseudocholinesterase activity of 1 U/mL at 37 ̊C.
Protein	> 0.1 mg protein/mg (A_280nm, E = 1.36)
Specific Activity	> 300 U/mg protein (Typically > 900 U/mg protein)
Appearance	White to pale, grey-green powder
Reconstitution	Reconstitute at 5 mg/mL using 50 mM Tris-HCl pH 7.3 - 7.5.
Solubility	Clear, colorless to slightly green solution (1 mg/mL in 0.9% saline)
Related Products	135-10 - BCHE from human serum 343-10 - Esterase from porcine liver
Storage	-20°C
Recertification	4 years
Synonyms	BCHE, BuChE, PCHE, pseudocholinesterase, plasma cholinesterase, Acylcholine acyl-hydrolase, Choline esterase, butyryl
Application	Diagnostic Manufacturing, Clinical Research
CAS Number	9001-08-5
E.C. Number	3.1.1.8
Gene	BCHE
Accession No	P06276 (human, 29 – 602), Q9N1N9 (equine)
Reaction	Acylcholine + H₂O = choline + carboxylic acid
Kinetics	K_M=18 µM (butyrylthiocholine, 25 degrees Celsius)
Activators	Ca²⁺
Inhibitors	Hg²⁺, EDTA, diisopropylfluorophosphate
Optimum pH	7 - 8