Creatine Kinase BB (CK-BB)

Product Name Creatine Kinase BB
Source Porcine Brain
Catalogue Number 191-24
Purity Purified
Form Lyophilized
Activity > 100 U/mg (Dimension® Clinical Chemistry System)
Unit Definition One Unit will transfer one micromole of phosphate from creatine phosphate to ADP per minute @ 37°C. Measured at 340 nm as one equimolar amount of NADH produced by a coupled reaction.
Protein > 0.6 mg protein/mg (Coomassie)
Specific Activity Reported in U/mg protein
Isoenzyme Content CK-BB: > 95% (Helena QuickGel® CK)
CK-MB: < 3%
CK-MM: < 3%
Appearance Tan to off-white powder
Solubility Clear, light yellow solution (1 mg/mL saline)
Formulation Lyophilized from 10 mM tris-succinate, 2 mM DTT, 2 mM EDTA, pH 6.5.
Related Products 190-24 - CK-MB from human heart
190-29 - CK-MM from human skeletal muscle
191-40 - Creatine Kinase from porcine heart
Recertification 3 years
Storage -20°C
Synonyms CPK-1, CKBB isoenyzme, CK-BB
CAS Number 9001-15-4
E.C. Number 2.7.3.2
Gene CKB
Accession No Q29594