Lee Biosolutions

Availability	Discontinued (can be custom produced). See product #190-29 for a suitable alternative.
Product Name	Creatine Kinase MM
Source	Human Skeletal Muscle
Catalogue Number	190-28
Purity	Highly Purified
Form	Lyophilized
Activity	> 100 U/mg (Dimension® Clinical Chemistry System)
Unit Definition	One unit will catalyze the transphosphorylation of one micromole of phosphate from creatine phosphate to ADP per minute at 37°C.
Protein	> 0.5 mg protein/mg (Coomassie)
Specific Activity	> 500 U/mg protein
Isoenzyme Content	CK-MM: > 99% (Helena QuickGel® CK) CK-MB: < 1% CK-BB: < 1%
Contaminants	LDH: < 0.01% (Dimension® Clinical Chemistry System) AST/GOT: < 0.01%
Appearance	White to off-white powder
Reconstitution	Reconstitute with tris buffered saline containing 1 mM DTT and 1% BSA. Centrifuge briefly to ensure complete recovery. Store up to 24 hours at 2 – 8˚C or up to three months at -20˚C. Avoid freeze-thaw cycles.
Formulation	Lyophilized from tris-succinate, EDTA and DTT, pH 7.5.
Related Products	190-24 - CK-MB from human heart 191-25 - CK-BB from human brain 191-40 - Creatine Kinase from porcine heart
Storage	-20°C
Recertification	3 years
Synonyms	CK-MM, CKMM
CAS Number	9001-15-4
E.C. Number	2.7.3.2
Gene	CKM, CKMM
Gene ID	1158