Creatine Kinase MM (CK-MM)

Product Name Creatine Kinase MM
Source Human Skeletal Muscle
Catalogue Number 190-28
Purity Highly Purified
Form Lyophilized
Activity > 100 U/mg (Dimension® Clinical Chemistry System)
Unit Definition One unit will catalyze the transphosphorylation of one micromole of phosphate from creatine phosphate to ADP per minute at 37°C.
Protein > 0.5 mg protein/mg (Coomassie)
Specific Activity > 500 U/mg protein
Isoenzyme Content CK-MM: > 99% (Helena QuickGel® CK)
CK-MB: < 1%
CK-BB: < 1%
Contaminants LDH: < 0.01% (Dimension® Clinical Chemistry System)
AST/GOT: < 0.01%
Appearance White to off-white powder
Reconstitution Reconstitute with tris buffered saline containing 1 mM DTT and 1% BSA.
Centrifuge briefly to ensure complete recovery. Store up to 24 hours at 2 – 8˚C or up to three months at -20˚C.
Avoid freeze-thaw cycles.
Formulation Lyophilized from tris-succinate, EDTA and DTT, pH 7.5.
Related Products 190-24 - CK-MB from human heart
191-25 - CK-BB from human brain
191-40 - Creatine Kinase from porcine heart
Storage -20°C
Recertification 3 years
Synonyms CK-MM, CKMM
CAS Number 9001-15-4
E.C. Number 2.7.3.2
Gene CKM, CKMM
Gene ID 1158