Alkaline Phosphatase (ALP/PLAP)

Catalogue Number: 485-10
Description
Product Name Alkaline Phosphatase
Source Human Placenta
Catalogue Number 485-10
Purity Purified
Form Lyophilized
Activity > 10 U/mg (Dimension® Clinical Chemistry System)
Unit Definition One unit will catalyze the hydrolysis of one micromole of p-nitrophenyl phosphate per minute at 37°C and pH 10.4 in glycine buffer.
Protein > 0.1 mg protein/mg (Coomassie)
Specific Activity Typically > 25 U/mg protein
Formulation Powder, lyophilized from tris chloride, with magnesium chloride and zinc chloride, pH 7.4.
Reconstitution Tris buffered saline containing 10 mg/mL BSA, 1 mM magnesium chloride, and 0.2 mM zinc chloride, pH 8.0. 1 – 10 mg/mL and dilute as needed.
Related Products 480-20 - Alkaline Phosphatase from calf intestines
490-20 - Alkaline Phosphatase from porcine kidney
270-60 - Ferritin from human placenta
Storage -20°C
Recertification 3 years
Specificity Tumor Marker, seminoma, ovarian cancer
Synonyms ALP, PLAP, Alk Phos
CAS Number 9001-78-9
E.C. Number 3.1.3.1
Overview

Lee Biosolutions is the leading supplier of Human Placental Alkaline Phosphatase (ALP) for life science, laboratory, research and diagnostic manufacturing uses.

Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.

Alkaline Phosphatase (ALP) is a hydrolase enzyme responsible for removing phosphate groups in the 5- and 3- positions from many types of molecules, including nucleotides, proteins, and alkaloids. In humans, alkaline phosphatase is present in all tissues throughout the entire body, but is particularly concentrated in liver, bile duct, kidney, bone, and the placenta. The optimal pH for the enzyme activity is pH=10 in standard conditions.

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SKU PRICE (USD) UNIT QTY
485-10-100 $ 100.00 100 units
485-10-1,000 $ 415.00 1,000 units
485-10-5,000 $ 1,575.00 5 x 1,000 units