Alpha-Amylase - DFP Treated

Catalogue Number: 122-18
Product Name Alpha-Amylase, DFP Treated
Source Porcine Pancreas
Catalogue Number 122-18
Purity Purified
Form Lyophilized
Product Details Alpha-Amylase (122-10) was treated with DFP (diisopropylfluorophosphate) to inactivate serine proteases.
Activity > 70 U/mg (Dimension® Clinical Chemistry System)
Unit Definition One unit will catalyze the hydrolysis of one micromole of 2-chloro-4-nitrophenyl-a-D-maltotrioside to yield 2-chloro-4-nitrophenol per minute at 37°C.
Protein > 0.4 mg protein/mg (Coomassie)
Specific Activity > 100 U/mg protein
Contaminants Lipase: < 5%
Protease: < 1%
Ammonia: < 0.1 micromole/mg
Formulation Lyophilized from 20 mM tris, 150 mM sodium chloride, and 3 mM calcium chloride, pH 7.5.
Appearance Off-white to white powder
Related Products 400-10 - Lipase from porcine pancreas
197-25 - Colipase from porcine pancreas
122-10 - α-Amylase from porcine pancreas
Storage -20°C
Recertification 3 years
Synonyms 1,4-alpha-D-Glucan-glucanohydrolase, amy2, PPA, PA
Molecular Weight ~54,000
CAS Number 9000-90-2
E.C. Number
Gene AMY2
Gene ID 397397
Accession No P00690

Lee Biosolutions produces porcine pancreatic alpha-Amylase for clinical research and diagnostic manufacturing.

Custom preparations, technical support, bulk quantities and aliquoting available, email for more details.

Unit Definition: One unit will catalyze the hydrolysis of one micromole of maltopentaose, which through coupled reactions results in the formation of 5 micromoles of glucose=6-phosphate per minute at 37C. Measured at 340 nm as an equimolar amount of NADH produced by a coupled reaction.

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122-18-5 $ 86.00 5 kU
122-18-25 $ 306.00 25 kU