Alpha-Amylase

Product Name	Alpha-Amylase
Source	Human Saliva
Catalogue Number	120-17
Purity	> 90% (SDS-PAGE)
Purity Note	Typically > 98% (SDS-PAGE, two bands at approx. 60kDa)
Form	Liquid
Activity	> 2,000 U/mL (Siemens Dimension® Clinical Chemistry System)
Unit Definition	One unit will catalyze the hydrolysis of one micromole 2-chloro-4-nitrophenyl-a-D-maltotrioside to yield 2-chloro-4-nitrophenol per minute at 37°C.
Protein	> 1.0 mg protein/mL (Coomassie)
Specific Activity	> 200 U/mg protein
Contaminants	Lipase: < 0.05% Protease: < 0.1% Calcium: < 3 mg/MU
Formulation	0.2 micron filtered solution in 10 mM Trizma® chloride, pH 7.5.
Appearance	Clear, colorless liquid
Related Products	120-10 - α-Amylase from human saliva - Lyophilized 400-20 - Lipase from human pancreas 122-40 - β-Amylase from sweet potato
Storage	-20°C
Recertification	1 year
Infectious Disease Testing	Negative or non-reactive at the donor level for anti-HIV 1 and 2, anti-HCV, HBsAg, HIV-1 (NAT), HBV (NAT), HCV (NAT), and syphilis by FDA approved methods.
Synonyms	1,4-alpha-D-Glucan-glucanohydrolase, ptyalin
Molecular Weight	~60,000
CAS Number	9000-90-2
E.C. Number	3.2.1.1
Gene	AMY1A
Gene ID	276
Accession No	P04745
References	Journal of Biosciences, 2013, Volume 38, Number 2, Page 259