Alanine Aminotransferase (ALT/GPT)

Product Name	Alanine Aminotransferase
Source	Porcine Heart
Catalogue Number	310-20
Purity	Purified
Form	Liquid
Activity	1,600 - 2,400 U/mL (Dimension® Clinical Chemistry System)
Unit Definition	One unit will catalyze the transamination of one micromole per minute of L-alanine to a-ketoglutarate, forming L-glutamate and pyruvate, at 37°C and pH 7.4.
Protein	Reported in mg protein/mL (Coomassie)
Specific Activity	Reported in U/mg protein
Contaminants	CPK: < 0.1% GLDH: < 0.01% AST/GOT: < 0.5% LDH: < 0.1% Protease: < 0.02 U/mg (< 0.001 O.D./8 min.)
Appearance	Yellow to green suspension
Formulation	Suspension in 1.2 M ammonium sulfate.
Related Products	310-30 - Alanine Aminotransferase (Lyophilized) from porcine heart 490-20 - Alkaline Phosphatase from porcine kidney 300-20 - Aspartate Aminotransferase from porcine heart
Storage	2-8°C
Recertification	2 years
Molecular Weight	~100,000
Product Specificity	Liver damage or disease
Synonyms	Glutamate Pyruvate Transaminase, Alanine Transaminase, serum glutamic-pyruvic transaminase, SGPT, GPT, ALT, ALAT
CAS Number	9000-86-6
E.C. Number	2.6.1.2
Gene	GPT, AAT1, GPT1