Alanine Aminotransferase (ALT/GPT)

Product Name	Alanine Aminotransferase
Source	Porcine Heart
Catalogue Number	310-30
Purity	Purified
Form	Lyophilized
Activity	> 90 U/mg (Dimension® Clinical Chemistry System)
Unit Definition	One unit will catalyze the transamination of one micromole per minute of L-alanine to a-ketoglutarate, forming L-glutamate and pyruvate, at 37°C and pH 7.4.
Protein	> 0.8 mg protein/mg solid (A₂₈₀)
Specific Activity	> 80 U/mg protein
Contaminants	CPK: < 0.1% GLDH: < 0.01% AST/GOT: < 0.5% LDH: < 0.1% Protease: < 0.02 U/mg (< 0.001 O.D./8 min.) Ammonia: < 0.5 micromole/mg
Formulation	Lyophilized from tris-chloride, pH 7.0 with sodium chloride, EDTA, DTT, a-ketoglutarate and pyridoxal-5-phosphate.
Appearance	Yellow to yellow-green powder
Solubility	Clear, colorless to yellow-green solution (1 mg/mL saline)
Reconstitution	Clear, yellow-green (10 mg/mL saline)
Related Products	480-20 - Alkaline Phosphatase from calf intestine 300-20 - Aspartate Aminotransferase from porcine heart 350-50 - Lactate Dehydrogenase from chicken heart
Storage	-20°C
Recertification	3 years
Molecular Weight	~100,000
Product Specificity	Liver damage or disease
Synonyms	Glutamate Pyruvate Transaminase, Alanine Transaminase, serum glutamic-pyruvic transaminase, SGPT, GPT, ALT, ALAT
CAS Number	9000-86-6
E.C. Number	2.6.1.2
Gene	GPT, AAT1, GPT1
Accession No	P13191