Alanine Aminotransferase (ALT/GPT)

Product Name Alanine Aminotransferase
Source Porcine Heart
Catalogue Number 310-30
Purity Purified
Form Lyophilized
Activity > 90 U/mg (Dimension® Clinical Chemistry System)
Unit Definition One unit will catalyze the transamination of one micromole per minute of L-alanine to a-ketoglutarate, forming L-glutamate and pyruvate, at 37°C and pH 7.4.
Protein > 0.8 mg protein/mg solid (A280)
Specific Activity > 80 U/mg protein
Contaminants CPK: < 0.1%
GLDH: < 0.01%
AST/GOT: < 0.5%
LDH: < 0.1%
Protease: < 0.02 U/mg (< 0.001 O.D./8 min.)
Ammonia: < 0.5 micromole/mg
Formulation Lyophilized from tris-chloride, pH 7.0 with sodium chloride, EDTA, DTT, a-ketoglutarate and pyridoxal-5-phosphate.
Appearance Yellow to yellow-green powder
Solubility Clear, colorless to yellow-green solution (1 mg/mL saline)
Reconstitution Clear, yellow-green (10 mg/mL saline)
Related Products 480-20 - Alkaline Phosphatase from calf intestine
300-20 - Aspartate Aminotransferase from porcine heart
350-50 - Lactate Dehydrogenase from chicken heart
Storage -20°C
Recertification 3 years
Molecular Weight ~100,000
Product Specificity Liver damage or disease
Synonyms Glutamate Pyruvate Transaminase, Alanine Transaminase, serum glutamic-pyruvic transaminase, SGPT, GPT, ALT, ALAT
CAS Number 9000-86-6
E.C. Number
Gene GPT, AAT1, GPT1
Accession No P13191