Leukocytes, Sonicated

Catalogue Number: 342-10
Description
Product Name Sonicated Leukocytes
Source Human Leukocytes
Catalogue Number 342-10
Form Liquid
Activity 5.0 - 6.0 U/mL (Elastase)
Unit Definition One unit will catalyze the hydrolysis of one micromole of methoxy succinyl-alanine-alanine-proline-valine-p-nitroanilide per minute at 37C and pH 7.5.
Protein > 1.0 mg/mL (Coomassie)
Dipstick Assay Leukocytes: > 2+
Protein: Negative
Glucose: Negative
Blood: Less than trace
Dipstick Protocol 0.9 mL per liter of saline will give a positive reaction for leukocytes with Roche Chemstrip® 10 SG and/or Siemens Multistix® 10 SG test strips.
Formulation Suspension in 154 mM sodium chloride.
Appearance Milky white suspension. May form aggregates due to freeze/thaws.
Handling Sonicate prior to use to disperse aggregates.
Storage -20°C
Recertification 2 years
Infectious Disease Testing Negative or non-reactive at the donor level for anti-HIV 1 and 2, anti-HCV, HBsAg, HIV-1 (NAT), HBV (NAT), HCV (NAT), and syphilis by FDA approved methods.
Synonyms Immune cells, LE, Elastase
Overview

Lee Biosolutions is the leading supplier of Human Sonicated Leukocytes (Elastase) for urinalysis manufacturing and research uses.

Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details. Single lots from 5 L available.

0.9 mL per liter of urine will give a positive reaction for leukocytes with a Siemens Multistix 10 or Roche Chemstrip 10 dipstick.

Human leukocyte esterase a urine test for presence of white blood cells abnormalities associated with infection. Leukocyte urine tests reveal the presence of granulocyte esterases, which cleave a derivatized pyrazole amino acid ester to liberate derivatized hydroxy pyrazole. This pyrazole then reacts with a diazonium salt to produce a purple color.

Leukocyte is a general term for a white blood cell. Human Leukocyte(s) include lymphocytes, polymorphonuclear leukocytes and monocytes

White blood cells in the urine usually indicate a urinary tract infection. Human leukocyte esterase (LE) tests detect esterase, an enzyme released by white blood cells. Human White blood cells are produced in the bone marrow and help to defend the body against infectious disease and foreign materials as part of the immune system. Human Leukocytosis is very common in acutely ill patients. It occurs in response to a wide variety of conditions, including viral, bacterial, fungal, or parasitic infection, cancer, and exposure to certain medications or chemicals.

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SKU PRICE (USD) UNIT QTY
342-10-1 $ 79.00 1 mL
342-10-5 $ 263.00 5 mL
342-10-25 $ 1,050.00 25 mL