Butyrylcholinesterase (BChE)

Catalogue Number: 130-10
Description
Product Name Butyrylcholinesterase
Source Equine Serum
Catalogue Number 130-10
Purity Highly Purified
Form Lyophilized
Activity > 50 U/mg (Siemens Dimension® Clinical Chemistry System)
Unit Definition One unit will catalyze the hydrolysis of one micromole of butyrylthiocholine to thiocholine and butyrate per minute at 37°C.
Protein > 0.1 mg protein/mg (A280nm, E = 1.36)
Specific Activity > 300 U/mg protein (Typically > 900 U/mg protein)
Appearance White to pale, grey-green powder
Reconstitution Reconstitute at 5 mg/mL using 50 mM Tris-HCl pH 7.3 - 7.5.
Solubility Clear, colorless to slightly green solution (1 mg/mL in 0.9% saline)
Related Products 135-10 - Butyrylcholinesterase from human serum
343-10 - Esterase from porcine liver
Storage 2-8°C
Recertification 4 years
Synonyms BCHE, BuChE, PCHE, pseudocholinesterase, plasma cholinesterase, Acylcholine acyl-hydrolase, Choline esterase, butyryl
Application Diagnostic Manufacturing, Clinical Research
CAS Number 9001-08-5
E.C. Number 3.1.1.8
Gene BCHE
Accession No P06276 (human, 29 – 602), Q9N1N9 (equine)
Reaction Acylcholine + H2O = choline + carboxylic acid
Kinetics KM=18 µM (butyrylthiocholine, 25 degrees Celsius)
Activators Ca2+
Inhibitors Hg2+, EDTA, diisopropylfluorophosphate
Optimum pH 7 - 8
Overview

Lee Biosolutions is the leading supplier of horse serum Butyrylcholinesterase (BCHE) for research and diagnostic manufacturing.

Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.

Butyrylcholinesterase (BCHE, or BuChE), is a non-specific cholinesterase enzyme that hydrolyses many different choline esters. In humans, it is found primarily in the liver and is encoded by the BCHE gene.

It is very similar to the neuronal acetylcholinesterase, which is also known as RBC or erythrocyte cholinesterase. The term "serum cholinesterase" is generally used in reference to a clinical test that reflects levels of both of these enzymes in the blood. Assay of butyrylcholinesterase activity in plasma can be used as a liver function test as both hypercholinesterasemia and hypocholinesterasemia indicate pathological processes.

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SKU PRICE (USD) UNIT QTY
130-10-1 $ 121.00 1 kU
130-10-5 $ 394.00 5 kU
130-10-15 $ 945.00 15 kU